翻訳と辞書 Words near each other ・ Phosphoribosylaminoimidazolesuccinocarboxamide synthase ・ Phosphoribosylanthranilate isomerase ・ Phosphoribosylformylglycinamidine synthase ・ Phosphoribosylglycinamide formyltransferase ・ Phosphoribosyltransferase ・ Phosphoribulokinase ・ Phosphoric ・ Phosphoric acid ・ Phosphoric acid fuel cell ・ Phosphoric acids and phosphates ・ Phosphoric monoester hydrolases ・ Phosphorine ・ Phosphoadenylyl-sulfate reductase (thioredoxin) ・ Phosphoadenylylsulfatase ・ Phosphoamidase ・ Phosphoamino acid analysis ・ Phosphocarrier protein ・ Phosphocholine ・ Phosphocreatine ・ Phosphodiester bond ・ Phosphodiesterase ・ Phosphodiesterase 2 ・ Phosphodiesterase 3 ・ Phosphodiesterase 4 ・ Phosphodiesterase I ・ Phosphodiesterase inhibitor ・ Phosphodiesterase-4 inhibitor ・ Phosphoenolpyruvate carboxykinase ・ Phosphoenolpyruvate carboxykinase (ATP) ・ Phosphoenolpyruvate carboxykinase (diphosphate) Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Phosphoamino acid analysis ： ウィキペディア英語版 Phosphoamino acid analysis Phosphoamino acid analysis, or PAA, is an experimental technique used in molecular biology to determine which amino acid or acids are phosphorylated in a protein. ==Technique== A protein is first phosphorylated using 32P-labeled ATP, usually via an ''in vitro'' kinase assay. Most of the amino acids in the protein are then hydrolyzed, usually by the use of a strong acid such as hydrochloric acid. These amino acids are then separated using 2-dimensional thin layer chromatography, along with amino acid standards for the three amino acids that are phosphorylated in eukaryotes: serine, threonine, and tyrosine. These amino acid standards can be visualized on the TLC substrate by exposure to ninhydrin, which colors the amino acids a visible purple when heated at ~100 °C. The radioactive amino acids can be detected via autoradiography, and an overlay of the two images will show which amino acids are phosphorylated. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Phosphoamino acid analysis」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.